3IUF
Crystal structure of the C2H2-type zinc finger domain of human ubi-d4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-08-06 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.26514 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 24.714, 57.657, 22.585 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 22.720 - 1.800 |
R-factor | 0.195 |
Rwork | 0.193 |
R-free | 0.23700 |
Structure solution method | MAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.491 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELX |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.000 | 40.000 | 1.830 |
High resolution limit [Å] | 1.800 | 4.880 | 1.800 |
Rmerge | 0.118 | 0.068 | 0.522 |
Number of reflections | 3263 | ||
<I/σ(I)> | 7.7 | ||
Completeness [%] | 99.9 | 100 | 98.7 |
Redundancy | 8.2 | 7.5 | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 9.5 | 291 | 30% PEG 3000, 0.1M CHES, protein concentration 5mg/mL., pH 9.5, vapor diffusion, temperature 291K |
1 | VAPOR DIFFUSION | 9.5 | 291 | 30% PEG 3000, 0.1M CHES, protein concentration 5mg/mL., pH 9.5, vapor diffusion, temperature 291K |