3IU8
M. tuberculosis methionine aminopeptidase with Ni inhibitor T03
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Collection date | 2009-06-19 |
| Wavelength(s) | 1 |
| Spacegroup name | P 63 |
| Unit cell lengths | 105.728, 105.728, 50.427 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.490 - 1.850 |
| R-factor | 0.167 |
| Rwork | 0.165 |
| R-free | 0.20000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yj3 |
| RMSD bond length | 0.028 |
| RMSD bond angle | 1.968 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.880 |
| High resolution limit [Å] | 1.850 | 5.020 | 1.850 |
| Rmerge | 0.156 | 0.055 | 0.714 |
| Number of reflections | 27549 | ||
| <I/σ(I)> | 6.2 | ||
| Completeness [%] | 99.5 | 99.9 | 90.5 |
| Redundancy | 11.1 | 10.6 | 8.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 0.1 M Bis-Tris, pH 5.5, 1.4 M AMS, 15% glycerol, vapor diffusion, hanging drop, temperature 298K |
