3IT5
Crystal Structure of the LasA Virulence Factor from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-09-09 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.488 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 105.818, 34.079, 105.837 |
| Unit cell angles | 90.00, 102.04, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.000 |
| R-factor | 0.19 |
| Rwork | 0.186 |
| R-free | 0.26200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.090 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 20.000 | 2.080 |
| High resolution limit [Å] | 2.010 | 4.320 | 2.010 |
| Rmerge | 0.062 | 0.048 | 0.119 |
| Number of reflections | 48468 | ||
| <I/σ(I)> | 11.8 | ||
| Completeness [%] | 96.4 | 97.5 | 90.7 |
| Redundancy | 3.2 | 3.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 277 | 50mM TrisCl 100mM NaCl, pH 7.0, spontaneous crystallization, temperature 277K |
