3IT1

Crystal Structure Francisella tularensis histidine acid phosphatase complexed with L(+)-tartrate

Replaces:  2GLA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2005-03-07
Detector	NOIR-1
Wavelength(s)	1.12711
Spacegroup name	P 41
Unit cell lengths	61.640, 61.640, 211.320
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.688 - 1.691
R-factor	0.185
Rwork	0.184
R-free	0.20700
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.029
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	SOLVE
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.510	1.760
High resolution limit [Å]	1.691	1.691
Rmerge	0.067	0.384
Total number of observations		19664
Number of reflections	79753
<I/σ(I)>	12.7	2.2
Completeness [%]	91.7	84.6
Redundancy	3.29	2.66

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	10% (w/v) Tacsimate, 0.1 M HEPES pH 7.0, 19% (w/v) PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 293K