3ISU

Crystal structure of the RGC domain of IQGAP3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2009-08-05
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97711
Spacegroup name	P 32 2 1
Unit cell lengths	58.120, 58.120, 78.292
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.000 - 1.880
R-factor	0.209
Rwork	0.207
R-free	0.24400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3iez
RMSD bond length	0.017
RMSD bond angle	1.612
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	1.910
High resolution limit [Å]	1.880	5.100	1.880
Rmerge	0.086	0.042	0.942
Number of reflections	12943
<I/σ(I)>	10.3
Completeness [%]	100.0	100	100
Redundancy	20.5	18.9	18.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	25% PEG3350, 0.2M lithium sulfate, 0.1 Bis-Tris, pH 6.5, vapor diffusion, sitting drop, temperature 291K