3IQL
Crystal structure of the rat endophilin-A1 SH3 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-02 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97720 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 64.338, 34.156, 62.729 |
| Unit cell angles | 90.00, 107.79, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.400 |
| R-factor | 0.12837 |
| Rwork | 0.127 |
| R-free | 0.15358 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gcq chain A Non-conserved residue pruned to CB atom using CHAINSAW |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.402 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.500 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.031 | 0.071 |
| Number of reflections | 23667 | |
| <I/σ(I)> | 30.2 | 11.1 |
| Completeness [%] | 96.3 | 85.5 |
| Redundancy | 3 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 277 | 20% methyl-2,4-pentanediol, 0.1M sodium acetate, 0.05M calcium chloride, 10mM 2-mercaptoethanol, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
