3IQF
Structure of F420 dependent methylene-tetrahydromethanopterin dehydrogenase in complex with methenyl-tetrahydromethanopterin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-03 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.008 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 103.400, 167.100, 122.500 |
| Unit cell angles | 90.00, 113.60, 90.00 |
Refinement procedure
| Resolution | 112.500 - 2.100 |
| R-factor | 0.16246 |
| Rwork | 0.160 |
| R-free | 0.20028 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qv9 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.631 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 112.500 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 246709 | |
| <I/σ(I)> | 14.9 | 2.7 |
| Completeness [%] | 99.2 | 77.6 |
| Redundancy | 4 | 77.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 30% PEG400, 0.1M MES, 0.1M sodium acetate, pH6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291.0K |
