3IQ7
Crystal Structure of human Haspin in complex with 5-Iodotubercidin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-11-03 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 69.034, 78.486, 87.048 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.150 - 2.000 |
| R-factor | 0.174 |
| Rwork | 0.172 |
| R-free | 0.20700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vuw |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.554 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.2) |
| Phasing software | PHASER (2.1.2) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.150 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.085 | 0.620 |
| Number of reflections | 32676 | |
| <I/σ(I)> | 10.6 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.4M (NH4)H2PO4, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
