3IO4
Huntingtin amino-terminal region with 17 Gln residues - Crystal C90
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.55 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 162.950, 101.210, 142.050 |
| Unit cell angles | 90.00, 90.42, 90.00 |
Refinement procedure
| Resolution | 37.420 - 3.630 |
| R-factor | 0.25397 |
| Rwork | 0.251 |
| R-free | 0.30354 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ior |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.180 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.000 |
| High resolution limit [Å] | 3.000 |
| Number of reflections | 23000 |
| <I/σ(I)> | 5.5 |
| Completeness [%] | 90.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 277 | 12% polyethylene glycol, 200 mM Zn Acetate, 200 mM Sodium Acetate, 100 mM Sodium Cacodylate pH 6.5 to 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
