3INZ
H57T Hfq from Pseudomonas aeruginosa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X12 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X12 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-06-26 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 61.290, 71.160, 104.410 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.700 |
R-factor | 0.1527 |
Rwork | 0.149 |
R-free | 0.21790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1u1s |
RMSD bond length | 0.010 |
RMSD bond angle | 1.315 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.4_4)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.740 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.040 | 0.362 |
Number of reflections | 50597 | |
<I/σ(I)> | 27.9 | 3.9 |
Completeness [%] | 94.1 | 80.4 |
Redundancy | 6.7 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.5 | 295 | 4 mg/ml protein, 50 mM NaCl, 100 mM NH4Cl, 7,5% MMEPEG 2000, 50 mM Tris HCl, 10 mM CdCl2, pH 8.5, VAPOR DIFFUSION, temperature 295K |