3IMP
New crystal form of the C-terminal domain of Helicobacter pylori MotB (residues 125-256)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-13 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 107.560, 100.336, 108.490 |
| Unit cell angles | 90.00, 119.51, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.500 |
| R-factor | 0.189 |
| Rwork | 0.186 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cyp |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.429 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.105 | 0.363 |
| Number of reflections | 69194 | |
| <I/σ(I)> | 10.4 | 2.8 |
| Completeness [%] | 99.0 | 99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 15% PEG3350, 200mM sodium tartrate, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
