3ILN
X-ray structure of the laminarinase from Rhodothermus marinus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE D03B-MX1 |
| Synchrotron site | LNLS |
| Beamline | D03B-MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-01 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.427 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.218, 108.288, 64.588 |
| Unit cell angles | 90.00, 113.90, 90.00 |
Refinement procedure
| Resolution | 25.922 - 1.950 |
| R-factor | 0.1586 |
| Rwork | 0.157 |
| R-free | 0.18960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hyk |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.024 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.200 | 2.060 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.104 | 0.372 |
| Number of reflections | 46598 | |
| <I/σ(I)> | 5.9 | 1.9 |
| Completeness [%] | 97.8 | 94.9 |
| Redundancy | 4 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 6% PEG 3350, 120mM phosphate buffer, 50mM potassium phosphate, 2% ethanol, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
