3ILD
Structure of ORF157-K57A from Acidianus filamentous virus 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-14 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.93 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 64.757, 64.757, 85.743 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 22.000 - 3.100 |
| R-factor | 0.27335 |
| Rwork | 0.271 |
| R-free | 0.30932 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ii2 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.331 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.000 | 3.270 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.072 | 0.404 |
| Number of reflections | 4047 | |
| <I/σ(I)> | 29.2 | 6.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.6 | 12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 20% PEG 8000, 0.2M MgCl2, 0.1M Tris, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
