3IKO

Crystal structure of the heterotrimeric Sec13-Nup145C-Nup84 nucleoporin complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Wavelength(s)	1.14
Spacegroup name	P 21 21 21
Unit cell lengths	101.397, 194.050, 327.806
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 3.200
Rwork	0.234
R-free	0.27300
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.500
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.310
High resolution limit [Å]	3.200	3.200
Number of reflections	105020
<I/σ(I)>	16.1	2
Completeness [%]	96.5	75.9
Redundancy	7.5	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	PEG 20000, MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K