3IKM

Crystal structure of human mitochondrial DNA polymerase holoenzyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2008-06-30
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 32
Unit cell lengths	138.394, 138.394, 226.312
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.920 - 3.240
R-factor	0.284
Rwork	0.284
R-free	0.30300
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	2.100
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.2)

Data quality characteristics

	Overall
Low resolution limit [Å]	47.000
High resolution limit [Å]	3.240
Number of reflections	76584
Completeness [%]	99.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION			VAPOR DIFFUSION