3IJM
The structure of a restriction endonuclease-like fold superfamily protein from Spirosoma linguale.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-31 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.97950, 0.97992 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.770, 65.849, 68.917 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.420 - 1.700 |
| R-factor | 0.168 |
| Rwork | 0.166 |
| R-free | 0.19900 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.382 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.058 | 0.477 |
| Number of reflections | 33607 | |
| <I/σ(I)> | 10.1 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.2 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 279 | 0.1M NaCl, 0.1M Bis Tris pH6.5, 1.5M (NH4)2SO4, VAPOR DIFFUSION, SITTING DROP, temperature 279K |
