3IJF
Crystal structure of cytidine deaminase from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE D03B-MX1 |
| Synchrotron site | LNLS |
| Beamline | D03B-MX1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-02-07 |
| Detector | MAC Science DIP-3000 |
| Wavelength(s) | 1.437 |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 63.705, 75.338, 55.116 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.240 - 1.990 |
| R-factor | 0.19264 |
| Rwork | 0.190 |
| R-free | 0.24015 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fr5 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.161 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 22.270 |
| High resolution limit [Å] | 1.990 |
| Rmerge | 0.086 |
| Number of reflections | 9374 |
| <I/σ(I)> | 10 |
| Completeness [%] | 97.0 |
| Redundancy | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 100 mM HEPES and 4.3 M NaCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
