3IJD
Uncharacterized protein Cthe_2304 from Clostridium thermocellum binds two copies of 5-methyl-5,6,7,8-tetrahydrofolic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-23 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97958 |
| Spacegroup name | P 1 |
| Unit cell lengths | 51.581, 52.368, 60.918 |
| Unit cell angles | 94.68, 97.68, 90.57 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.221 |
| Rwork | 0.218 |
| R-free | 0.26500 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.405 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.9) |
| Phasing software | SHELXCD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.071 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.068 | 0.296 |
| Number of reflections | 40872 | |
| <I/σ(I)> | 10.2 | 3.3 |
| Completeness [%] | 96.0 | 95.6 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5 | 294 | 100mM Bis-Tris pH 5.0, 25% PEG 3350, 200mM magnesium chloride, vapor diffusion, temperature 294K |
