3IJ4
Cesium sites in the crystal structure of a functional acid sensing ion channel in the desensitized state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-08-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.3776 |
| Spacegroup name | H 3 |
| Unit cell lengths | 131.787, 131.787, 119.107 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.470 - 3.000 |
| R-factor | 0.225 |
| Rwork | 0.223 |
| R-free | 0.27200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.163 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.000 | 3.077 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.095 | 0.084 |
| Number of reflections | 15430 | |
| <I/σ(I)> | 1.1 | |
| Completeness [%] | 93.5 | 95.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277.15 | 150-350 mM NaCl, 100 mM HEPES, 23-28% PEG 400, 10 mM taurine, soaked in 250 mM CsCl cryo solution, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K |
