3IJ3
1.8 Angstrom Resolution Crystal Structure of Cytosol Aminopeptidase from Coxiella burnetii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-07 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 112.803, 112.803, 78.089 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.210 - 1.800 |
| R-factor | 0.14855 |
| Rwork | 0.147 |
| R-free | 0.17685 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lam |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.508 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.087 | 0.530 |
| Number of reflections | 53424 | |
| <I/σ(I)> | 24.3 | 4.6 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 8.9 | 8.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 295 | Protein solution at 7.0 mg/mL, 0.5M Sodium cloride, Screen solution JCSG+D7, 0.2M Lithium sulfate, 0.1M Tris, 40% v/v PEG 400, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
