3IIS
Structure of the reconstituted Peridinin-Chlorophyll a-Protein (RFPCP)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-06-17 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9797 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 68.690, 81.623, 75.080 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.850 - 1.400 |
R-factor | 0.15353 |
Rwork | 0.152 |
R-free | 0.18725 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ppr N-Domain of M monomer |
RMSD bond length | 0.017 |
RMSD bond angle | 3.339 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.900 | 1.500 |
High resolution limit [Å] | 1.400 | 1.400 |
Number of reflections | 40040 | |
<I/σ(I)> | 14.07 | 4.04 |
Completeness [%] | 95.7 | 92 |
Redundancy | 7.6 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 296 | 0.1M CdCl2, 0,1M Sodium Acetate pH 4.6, 20-24% PEG 400, VAPOR DIFFUSION, HANGING DROP, temperature 296K |