3IHR
Crystal Structure of Uch37
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-01 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97949, 0.97973 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 90.092, 98.736, 154.061 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.560 - 2.950 |
| R-factor | 0.201 |
| Rwork | 0.199 |
| R-free | 0.24200 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.092 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.850 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.078 | 0.470 |
| Number of reflections | 15698 | |
| <I/σ(I)> | 13.6 | 3.3 |
| Completeness [%] | 86.1 | 52.7 |
| Redundancy | 11.4 | 8.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | Total volume 4 ul; 5 mg/ml Uch37, 1.3 M sodium formate, 100 mM Tris, 2.5 mM BisTris, 0.15 mM TCEP, pH 8.5, hanging drop, batch, temperature 277K, VAPOR DIFFUSION, HANGING DROP |
