3IHL
Human CTPS2 crystal structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-30 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 168.250, 168.250, 132.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.910 - 2.800 |
| R-factor | 0.20527 |
| Rwork | 0.203 |
| R-free | 0.23232 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vo1 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.342 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.910 | 3.000 |
| High resolution limit [Å] | 2.800 | 2.800 |
| <I/σ(I)> | 29.76 | |
| Completeness [%] | 5.3 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.8M Succinic acid, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
