3IHE
Crystal structure of mouse Bcl-xl mutant (F105A) at pH 6.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 6C1 |
| Synchrotron site | PAL/PLS |
| Beamline | 6C1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-08 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 63.151, 63.151, 110.323 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.560 - 3.000 |
| R-factor | 0.19455 |
| Rwork | 0.192 |
| R-free | 0.23947 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pq0 |
| RMSD bond length | 0.049 |
| RMSD bond angle | 3.728 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.090 | 0.098 |
| Number of reflections | 4232 | |
| <I/σ(I)> | 16.3 | 3.3 |
| Completeness [%] | 91.5 | 50.7 |
| Redundancy | 9.4 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | 1.7M Ammonium sulphate, MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
