3IHD
Crystal structure of mouse Bcl-xl mutant (Y101A) at pH 5.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 6C1 |
| Synchrotron site | PAL/PLS |
| Beamline | 6C1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-08 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 62.811, 62.811, 111.137 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.240 - 1.880 |
| R-factor | 0.17302 |
| Rwork | 0.171 |
| R-free | 0.21043 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pq0 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.817 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.950 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmerge | 0.080 | 0.080 |
| Number of reflections | 16650 | |
| <I/σ(I)> | 18.9 | 1.6 |
| Completeness [%] | 93.5 | 51.2 |
| Redundancy | 10.6 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 295 | 1.4M Ammonium Sulphate, Tri-Na citrate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
