3IGY
Crystal structures of Leishmania mexicana phosphoglycerate mutase at high cobalt concentrations
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.870 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.746, 72.111, 129.946 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.011 - 2.003 |
| R-factor | 0.1568 |
| Rwork | 0.155 |
| R-free | 0.19900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.185 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.011 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 40289 | |
| Completeness [%] | 99.7 | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.3 | 290 | 80mM Ammonium acetate, 40mM trisodium citrate pH 6.0, 24%(w/v) PEG4000, VAPOR DIFFUSION, temperature 290K |
