3IGE
Small outer capsid protein (Soc) from bacteriophage RB69
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2008-04-15 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 39.220, 88.613, 81.944 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.595 - 2.254 |
R-factor | 0.2189 |
Rwork | 0.217 |
R-free | 0.26270 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | structure of the Soc protein from bacteriophage RB69 (hexagonal crystal form). |
RMSD bond length | 0.002 |
RMSD bond angle | 0.421 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 23.600 |
High resolution limit [Å] | 2.540 |
Number of reflections | 7033 |
<I/σ(I)> | 21.2 |
Completeness [%] | 99.6 |
Redundancy | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 30% PEG 8000; 0.1 M imidazole; 0.2 M NaCl., VAPOR DIFFUSION, HANGING DROP, temperature 277K |