3IFW
Crystal structure of the S18Y variant of ubiquitin carboxy terminal hydrolase L1 bound to ubiquitin vinylmethylester.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-27 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0032 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 87.304, 87.304, 193.544 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.400 |
| R-factor | 0.21105 |
| Rwork | 0.209 |
| R-free | 0.25627 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ETL PDB ENTRY 1XD3 CHAIN B |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.896 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.094 | 0.600 |
| Number of reflections | 11248 | |
| <I/σ(I)> | 21 | 3 |
| Completeness [%] | 98.5 | 99.8 |
| Redundancy | 9.9 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 298 | 2.4 M ammonium sulfate, 0.1M BICINE, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
