3IFV
Crystal structure of the Haloferax volcanii proliferating cell nuclear antigen
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-05-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97640 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 83.360, 143.760, 78.030 |
| Unit cell angles | 90.00, 121.56, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.000 |
| R-factor | 0.21683 |
| Rwork | 0.214 |
| R-free | 0.25931 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rwz |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.171 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.960 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.066 | 0.315 |
| Number of reflections | 52702 | |
| <I/σ(I)> | 11.6 | 3.3 |
| Completeness [%] | 95.3 | 95.9 |
| Redundancy | 2.5 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 283 | 0.2M calcium chloride, 0.1M HEPES pH 7.5, 18% PEG 4000, VAPOR DIFFUSION, SITTING DROP, temperature 283K |
