3IF4
Structure from the mobile metagenome of North West Arm Sewage Outfall: Integron Cassette Protein Hfx_Cass5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9798 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.324, 44.481, 82.462 |
| Unit cell angles | 90.00, 109.81, 90.00 |
Refinement procedure
| Resolution | 40.226 - 2.181 |
| R-factor | 0.1828 |
| Rwork | 0.178 |
| R-free | 0.23280 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.876 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.240 |
| High resolution limit [Å] | 2.180 | 2.200 |
| Rmerge | 0.093 | 0.531 |
| Number of reflections | 21618 | |
| <I/σ(I)> | 34.6 | 2.62 |
| Completeness [%] | 97.6 | 68.6 |
| Redundancy | 7 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294 | 29% PEG3350, 0.1 M Hepes pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
