Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL44B2 |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2007-03-17 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.2824 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 143.439, 149.738, 122.118 |
| Unit cell angles | 90.00, 110.61, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.800 |
| Rwork | 0.219 |
| R-free | 0.27500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2dkf |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 6.030 | 2.800 |
| Rmerge | 0.049 | 0.028 | 0.293 |
| Number of reflections | 55276 | ||
| <I/σ(I)> | 23.2 | ||
| Completeness [%] | 93.6 | 99.5 | 99.6 |
| Redundancy | 3.7 | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 1.0M Ammonium Sulfate, 1%(w/v) Polyethylene Glycol 3350, 0.1M Bis-Tris, 4%(v/v) 2,2,2 Trifluoroethanol, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
