3ID3
Crystal Structure of RseP PDZ2 I304A domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR-H |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-16 |
| Detector | BRUKER SMART 6000 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.411, 38.147, 51.265 |
| Unit cell angles | 90.00, 102.84, 90.00 |
Refinement procedure
| Resolution | 49.983 - 2.010 |
| R-factor | 0.1978 |
| Rwork | 0.197 |
| R-free | 0.22230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zpm |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.936 |
| Data reduction software | SAINT |
| Data scaling software | PROTEUM PLUS (PLUS) |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.983 | 2.110 |
| High resolution limit [Å] | 2.010 | 2.010 |
| Number of reflections | 10330 | |
| <I/σ(I)> | 9.85 | 3.21 |
| Completeness [%] | 85.2 | 85.2 |
| Redundancy | 1.55 | 0.61 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 4M Sodium Formate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
