3ICY
The crystal structure of sensory box histidine kinase/response regulator domain from Chlorobium tepidum TLS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-07-12 |
Detector | SBC-2 |
Wavelength(s) | 0.9794 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 106.728, 32.483, 93.755 |
Unit cell angles | 90.00, 114.67, 90.00 |
Refinement procedure
Resolution | 26.917 - 2.684 |
R-factor | 0.1711 |
Rwork | 0.167 |
R-free | 0.24830 |
Structure solution method | SAD |
Starting model (for MR) | ARP/wARP |
RMSD bond length | 0.008 |
RMSD bond angle | 1.115 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MLPHARE |
Refinement software | PHENIX (1.5_2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.750 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.086 | 0.260 |
Number of reflections | 8394 | |
<I/σ(I)> | 27.6 | 7 |
Completeness [%] | 99.8 | 99.7 |
Redundancy | 4.8 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 0.5M (NH4)2HPO4, 10% PEG400, Na-citrate, pH5.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |