3ICQ
Karyopherin nuclear state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-26 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.070 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 143.000, 143.800, 166.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.200 |
| Rwork | 0.243 |
| R-free | 0.29400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ibv |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.407 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.25) |
| Phasing software | PHASER (2.1.3) |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.174 | 3.370 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.149 | 0.650 |
| Total number of observations | 34787 | |
| Number of reflections | 57452 | |
| <I/σ(I)> | 10.1 | 2.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.2 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 100mM Bis-Tris, pH5.5, 20% PEG3350, 180mM sodium acetate at 18 degrees, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Bis-Tris | ||
| 2 | 1 | 1 | PEG3350 | ||
| 3 | 1 | 1 | sodium acetate | ||
| 4 | 1 | 1 | HOH | ||
| 5 | 1 | 2 | Bis-Tris | ||
| 6 | 1 | 2 | PEG3350 | ||
| 7 | 1 | 2 | sodium acetate | ||
| 8 | 1 | 2 | HOH |
