3IC9
The structure of dihydrolipoamide dehydrogenase from Colwellia psychrerythraea 34H.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-09 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97937, 0.97953 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.497, 246.353, 73.664 |
| Unit cell angles | 90.00, 90.20, 90.00 |
Refinement procedure
| Resolution | 43.900 - 2.150 |
| R-factor | 0.1885 |
| Rwork | 0.186 |
| R-free | 0.23338 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.427 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.000 | 2.190 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.107 | 0.460 |
| Number of reflections | 138959 | |
| <I/σ(I)> | 17.6 | 2.2 |
| Completeness [%] | 99.1 | 93.6 |
| Redundancy | 3.6 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 297 | 0.1M Bis-tris, 20%(w/v) PEG MME5000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
