3IC7
Crystal Structure of Putative Transcriptional Regulator of GntR Family from Bacteroides thetaiotaomicron
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 82.826, 82.826, 85.162 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.240 - 2.819 |
| R-factor | 0.237 |
| Rwork | 0.236 |
| R-free | 0.26300 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.387 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine: 1.4_58)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.240 | 2.870 |
| High resolution limit [Å] | 2.819 | 2.820 |
| Number of reflections | 8473 | |
| <I/σ(I)> | 8.3 | 2 |
| Completeness [%] | 99.7 | 96.7 |
| Redundancy | 11.5 | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.2 M ammonium acetate, 0.1 M HEPES pH 7.5, 45 % MPD, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
