3IC6
Crystal structure of putative methylase family protein from Neisseria gonorrhoeae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97940 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 73.123, 73.123, 193.315 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.590 |
| R-factor | 0.21039 |
| Rwork | 0.209 |
| R-free | 0.24433 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.496 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.620 |
| High resolution limit [Å] | 2.590 | 2.600 |
| Rmerge | 0.103 | 0.570 |
| Number of reflections | 10136 | |
| <I/σ(I)> | 40.1 | 4.76 |
| Completeness [%] | 99.3 | 99.2 |
| Redundancy | 9.9 | 10.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 297 | 8% PEG 4000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
