3IBV

Karyopherin cytosolic state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2008-04-24
Detector	PSI PILATUS 6M
Wavelength(s)	0.96
Spacegroup name	P 21 21 2
Unit cell lengths	119.900, 130.100, 173.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.900 - 3.100
Rwork	0.275
R-free	0.31300
Structure solution method	MIR
RMSD bond length	0.012
RMSD bond angle	1.606
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELXS
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.900		3.200
High resolution limit [Å]	3.100	10.000	3.100
Rmerge	0.092	0.033	0.750
Number of reflections	48907	1599	4107
<I/σ(I)>	17.22	44.8	2.3
Completeness [%]	97.7	97.2	92.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	100mM MES, pH6.5, 30% PEG400, 160mM CaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	100mM MES, pH6.5, 30% PEG400, 160mM CaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	100mM MES, pH6.5, 30% PEG400, 160mM CaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	100mM MES, pH6.5, 30% PEG400, 160mM CaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 298K