3IBG

Crystal structure of Aspergillus fumigatus Get3 with bound ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	CCD
Collection date	2008-12-14
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	67.760, 154.780, 242.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	65.264 - 3.200
R-factor	0.2139
Rwork	0.212
R-free	0.25130
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.038
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE (2.13)
Refinement software	PHENIX (1.4_62)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	65.268	3.370
High resolution limit [Å]	3.200	3.200
Number of reflections	43190
<I/σ(I)>	10.4	2.5
Completeness [%]	100.0	100
Redundancy	4.9	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	298	0.2 M potassium citrate, 16% PEG 3350, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K
1	VAPOR DIFFUSION, SITTING DROP	8	298	0.2 M potassium citrate, 16% PEG 3350, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K