3IB8
Crystal structure of full length Rv0805 in complex with 5'-AMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-28 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9864 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 100.400, 100.400, 80.801 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.750 - 1.800 |
| R-factor | 0.152 |
| Rwork | 0.150 |
| R-free | 0.16800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ib7 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.237 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.067 | 0.619 |
| Number of reflections | 38865 | |
| <I/σ(I)> | 9.7 | 3.8 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 8 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Bis-Tris, amm. acetate, MPD, AMP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
