3IAR
The crystal structure of human adenosine deaminase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-07-01 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54180 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.070, 73.510, 76.660 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.698 - 1.520 |
| R-factor | 0.1537 |
| Rwork | 0.153 |
| R-free | 0.18490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1krm |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.518 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.698 | 1.600 |
| High resolution limit [Å] | 1.520 | 1.520 |
| Rmerge | 0.048 | 0.251 |
| Number of reflections | 52538 | |
| <I/σ(I)> | 18.6 | 3.1 |
| Completeness [%] | 97.8 | 86.5 |
| Redundancy | 4.2 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 20% PEG 3350, 0.20M NaNO3, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
