3IAK
Crystal structure of human phosphodiesterase 4d (PDE4d) with papaverine.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 83.502, 83.502, 148.144 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.750 - 2.800 |
| R-factor | 0.24121 |
| Rwork | 0.239 |
| R-free | 0.29148 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qyk |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.906 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.750 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.090 | 0.386 |
| Number of reflections | 13523 | |
| <I/σ(I)> | 11 | 4.1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.87 | 8.15 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 278 | 2.5M ammonium sulphate, 100mM sodium citrate pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 278K |
