3IA6
X-ray Crystal structure of the nuclear hormone receptor PPAR-gamma in a complex with a PPAR gamma/alpha dual agonist
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2004-07-01 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 93.041, 62.187, 118.547 |
| Unit cell angles | 90.00, 102.66, 90.00 |
Refinement procedure
| Resolution | 33.000 - 2.310 |
| R-factor | 0.24126 |
| Rwork | 0.238 |
| R-free | 0.29712 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2q8s |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.133 |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.000 | 2.380 |
| High resolution limit [Å] | 2.310 | 2.310 |
| Number of reflections | 28605 | |
| <I/σ(I)> | 33 | 15 |
| Completeness [%] | 98.2 | 100 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 288 | well solution consisting of 0.6 - 0.8 M tri-sodium citrate, 0.1 M imidazole pH 8 and 1 mM TCEP, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
