3I90
Crystal structure of human chromobox homolog 6 (CBX6) with H3K27 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ DW |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS VII |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 53.421, 53.421, 227.778 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 2.000 |
| R-factor | 0.254 |
| Rwork | 0.252 |
| R-free | 0.30000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gv6 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.942 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 40.000 | 2.080 |
| High resolution limit [Å] | 2.000 | 4.950 | 2.000 |
| Rmerge | 0.100 | 0.056 | 0.634 |
| Number of reflections | 13855 | ||
| <I/σ(I)> | 14 | ||
| Completeness [%] | 99.7 | 97.8 | 99.6 |
| Redundancy | 17.1 | 10.2 | 19.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 2.5M (NH4)2SO4, 0.1 M Tris-HCl pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
