3I8X

Structure of the cytosolic domain of E. coli FeoB, GDP-bound form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I911-3
Synchrotron site	MAX II
Beamline	I911-3
Temperature [K]	100
Detector technology	CCD
Collection date	2007-09-29
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9085
Spacegroup name	P 1 21 1
Unit cell lengths	74.731, 56.058, 90.799
Unit cell angles	90.00, 91.70, 90.00

Refinement procedure

Resolution	25.140 - 2.250
R-factor	0.18946
Rwork	0.186
R-free	0.24965
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3i8s
RMSD bond length	0.015
RMSD bond angle	1.516
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.3.0037)

Data quality characteristics

	Overall
Low resolution limit [Å]	25.150
High resolution limit [Å]	2.250
Number of reflections	35991

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4.9	285	10 % PEG 4000, 100 mM Na-malonate, pH 4.9, VAPOR DIFFUSION, temperature 285K