3I8T
N-terminal CRD1 domain of mouse Galectin-4 in complex with lactose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-10-21 |
Detector | SBC-3 |
Wavelength(s) | 0.979 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 90.841, 90.841, 57.104 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.560 - 2.100 |
R-factor | 0.18874 |
Rwork | 0.187 |
R-free | 0.22413 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2gal |
RMSD bond length | 0.018 |
RMSD bond angle | 1.817 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.620 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.071 | 0.472 |
Number of reflections | 14348 | |
<I/σ(I)> | 7.6 | |
Completeness [%] | 99.2 | 97.5 |
Redundancy | 7.6 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291 | 0.1M NaCacodylate pH5.0, 15% PEG4000, 0.2M Ammonium Sulphate, 80mM lactose, protein concentration 9.6mg/ml, drop 1+1ul, microseeding, VAPOR DIFFUSION, HANGING DROP, temperature 291K |