3I8S

Structure of the cytosolic domain of E. coli FeoB, nucleotide-free form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I911-3
Synchrotron site	MAX II
Beamline	I911-3
Temperature [K]	100
Detector technology	CCD
Collection date	2007-06-21
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.1758, 0.9783, 0.9792, 0.8266
Spacegroup name	P 1 21 1
Unit cell lengths	74.400, 56.280, 90.620
Unit cell angles	90.00, 91.86, 90.00

Refinement procedure

Resolution	23.820 - 1.800
R-factor	0.17867
Rwork	0.177
R-free	0.21742
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.585
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	REFMAC (5.3.0037)

Data quality characteristics

	Overall
Low resolution limit [Å]	23.900
High resolution limit [Å]	1.800
Number of reflections	69615

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4.9	285	10 % PEG 4000, 100 mM Na-malonate, pH 4.9, VAPOR DIFFUSION, temperature 285K