3I87
Ethanolamine Utilization Microcompartment Shell Subunit, EutL Open Form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 67.799, 67.799, 80.520 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 80.580 - 2.300 |
| R-factor | 0.19 |
| Rwork | 0.188 |
| R-free | 0.23400 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.873 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 90.000 | 90.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 4.950 | 2.300 |
| Rmerge | 0.131 | 0.048 | 0.493 |
| Number of reflections | 8975 | ||
| <I/σ(I)> | 7.5 | ||
| Completeness [%] | 90.9 | 84.7 | 93.6 |
| Redundancy | 6 | 6 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 0.1M sodium/potassium phosphate, 2.2M sodium chloride, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
