3I7S
Dihydrodipicolinate synthase mutant - K161A - with the substrate pyruvate bound in the active site.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-10-10 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 120.881, 120.881, 110.500 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.170 - 2.300 |
| R-factor | 0.19784 |
| Rwork | 0.196 |
| R-free | 0.23582 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yxc |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.364 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.170 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.088 | 0.350 |
| Number of reflections | 39830 | |
| <I/σ(I)> | 7.4 | 2.8 |
| Completeness [%] | 95.0 | 97.3 |
| Redundancy | 3.42 | 3.27 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 10 | 276 | 1.8M POTASSIUM PHOSPHATE, PH 10, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 276K |
