3I78
35/99/170/186/220-loops of FXa in SGT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-04-10 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 113.710, 113.710, 52.616 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.940 - 3.000 |
| R-factor | 0.211 |
| Rwork | 0.208 |
| R-free | 0.27500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3beu |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.607 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.000 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.105 | 0.323 |
| Number of reflections | 7515 | |
| <I/σ(I)> | 11.8 | 2.6 |
| Completeness [%] | 97.6 | |
| Redundancy | 5.8 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 10.5 | 298 | 2.0 M Ammonium sulfate, 0.1 M CAPS, 75 mM NaCl, 25 mM Benzamidine, pH 10.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
